Herstellung polyklonaler Antikörper

polyklonale Antikörper

Genosphere Biotechnologies, Paris | Antikörperproduktionsservice | polyklonale Antikörper | Host - Kaninchen | Meerschweinchen | Ratte | Maus | Huhn | Ziege.

Seit 1997 stellt Genosphere Biotechnologies seinen Immunisierservice Kunden weltweit zur Verfügung. Polyklonale Antikörper im Kundenauftrag, generiert in Maus, Ratte, Meerschweinchen, Kaninchen, Ziege und Huhn - Quietly reliable.

Die Antikörper werden in den vier Reinheitsstufen: "crude", "total IgG", "affinity chromatography", "double affinity chromatography" angeboten.
Als Host stehen : mouse, guinea pig, rat, rabbit, chicken, goat zur Auswahl.

See also: Avian IgY antibodies (Hen)

Wake up call. Fordern Sie jetzt ein Preisangebot für Ihr Projekt an.

Quietly reliable - Peptidsyntheseservice und Antikörperproduktion aus einer Hand !

Antibodies development services

+ Polyclonal and Monoclonal

+ Anti-peptide, anti-protein

+ Phospho-specific antibody development

+ Hosts: goats, rabbit, guinea pig, rat, mouse, hen

+ Purification: protein A, affinity purified

+ High ELISA titer guarantees

anti-Peptide pAb development

Affinity purified antibody

examplified ELISA Result:

ABS 405 nm

Antibody

Purification method : affinity column

Rabbit No 1

dilution Pre-immune Purified

1:25 000 0.136 2.00
1:50 000 0.052 1.55
1:100 000 0.035 1.26

Rabbit No 2

dilution Pre-immune Purified

1:25 000 0.147 2.02
1:50 000 0.062 1.98
1:100 000 0.034 1.42
Interpretation of ELISA titer: < 1:1 000 = Below average ; 1:1 000 - 1:10 000 = Average ;
1:10 000 = Good; 1:10 000-100 000 = Excellent


ELISA Procedure:
Antigens are coated on EIA strips at 10μg per well. Wells are washed with 200 μl PBS buffer.
Antisera is diluted in series, added in separate wells , and incubated for 2 hours.
Unbound antibodies are washed and anti-Rabbit IgG-HRP conjugate is added.
Plates are washed and color development run for 15 min. with TMB substrate.
Absorbance is read at 405 nm (2.00 AUFS).
Color intensity is directly proportional to the amount of antibodies.
Antibody is positive if absorbance is >2 folds over that of pre-immune serum.
Background absorbance for pre-immune serum can reach 0.1 to 0.3

Pigeon Tektin-5 (TEKT5)

Multi-Peptide Co-Immunization

In some instances, in order to improve the chances of producing a successful antibodies pool, one may choose several target peptides within the same protein sequences and use them as antigen in a co-immunization protocol. Having several epitopes derived from a single protein will increase the chances of obtaining a pool of antibodies that will recognize the target protein. Some background signal increase may be experienced in some instances.

We will synthesize, purify and conjugate each peptide separately. Individual KLH conjugates are then mixed in equimolar ratios and used as immunogen.

anti-Peptide IgY : ab 956 €

Zugriffe heute: 3 - gesamt: 3370.

IgY chicken antibodies

50-100 mg total IgY purified antibodies derived from yolk (eggs). ELISA, guaranteed titer >1:20 000 from purified egg yolk.

Bestellnummer: HP1AB05-01



Chicken IgY Antibodies
In many instances, raising antibodies in chicken has proven essential. Where genetically distant species is required e.g. conserved mammalian proteins, Genosphere Biotechnologies will produce your antibodies in chicken. IgY antibodies may be isolated from circulating blood of chicken or extracted and purified in high yields from egg yolks of immunized laying hens.

Anti-peptide service offer:
• Synthesis, purification & KLH conjugation of your peptide
• Immunization of 2 hens
• Eggs collection, yolk isolation or antiserum collection and IgY purification
√ ELISA titered IgY antibodies fraction
√ Pre-immune purified IgY
√ Full report with ELISA analysis data
• 2 mg of unconjugated peptide (with HPLC & MS data, purity >90%)

Anti-protein service offer (you provide the antigen):
• Immunization of 2 hens
• Eggs collection, yolk isolation or antiserum collection and IgY purification
√ ELISA titered IgY antibodies fraction
√ Pre-immune purified IgY
√ Full report with ELISA analysis data

Pre-immune eggs are collected and control IgYs are purified. Following four immunizations over a 70-days period, eggs or antiserum are collected and we isolate the IgY fraction (purity 90-95%). The final antibodies preparation is then titered by ELISA. In addition, the total IgY fraction may be further purified by affinity chromatography using a peptide- or antigen-column to yield a specific hen antibody. For large scale IgY production, multiple hen immunization, and timely supply over long periods, please contact us with your project details and we will design the most efficient strategy for you.

Inquiries: info@genosphere-biotech.de