E.coli

Service description

• Codon optimisation and gene synthesis
• Subcloning of gene in T7 promoter-based expression vectors
• Transform construct into 3 different E coli strains
• Screening & expression pilot: cystoplasm or inclusion bodies
• Scale up expression and purification: 1-10 L fermentation
• Affinity purification (Ni2+, GST, etc)
• Refolding (inclusion bodies)
• QC: SDS-PAGE analysis, western blot, purity, concentration
• Project report

Lead time: 7-8 weeks

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