einfach und doppelt affinitätsgereinigte pAk (IgG)
Antigen-Präparation und Immunisierung
| - Synthesis, purification & KLH conjugation of your peptide for immunisation. |
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| - Delivery: Pre-immune control serum. Delivery: 2 mg of free peptide, purity >90% (with HPLC & MS data) |
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- Immunisation of 2 animals. Host: rabbit, guinea pig, rat, mouse, mixed composition of hosts upon special request. |
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- 4 immunisations (70 days protocol), 80-100 ml ELISA titered crude antiserum. Additional booster immunisation if of need. |
| - guaranteed titer of your antiserum at this point >1:20 000 |
Protein A/G Affinitätschromatographie
| -Preparation of affinity column by covalent attachment of Protein A/G |
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-Protein A/G-Affinity chromatography of your antiserum |
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-Quantitation and ELISA of Protein A/G purified total IgG antibodies |
| - Delivery: 2-5 mg purified total IgG, Full report with ELISA data, guaranteed titer >1:30:000 |
Peptidspezifische Affinitätschromatographie
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-Preparation of affinity column by covalent attachment of your peptide |
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-(your) antigenic peptide specific-Affinity chromatography of your antiserum |
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-Quantitation and ELISA of (your) specific antigenic peptide purified specific IgG antibodies |
| - Delivery: 2-5 mg purified specific IgG, Full report with ELISA data, guaranteed titer >1:40:000 |
Zugriffe heute: 3 - gesamt: 8767.