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Proteinexpressionsservice von Genosphere BT

Proteinexpression von Genosphere Biotechnologies.
Proteinexpression von Genosphere Biotechnologies. Wir synthetisieren Ihr Gen "100% richtig" und exprimieren das Protein in eu- und prokaryotischen Expressionssystemen. eMail: protein@genosphere-biotech.com

Nicht jedes Protein lässt sich gleich gut exprimieren und anschließend isolieren , daher bieten wir Ihnen verschiedene Expressionssysteme für die Herstellung ihrer Proteine an.

Expressionssysteme

E.coli
Yeast
Insect cells / Baculovirus
Mammalian cells

Using the right protein expression system for your specific application is critical to success. Consider protein solubility, functionality, purification speed, and yield when choosing an expression system. We offer a wide selection of superior mammalian, insect, yeast, and bacterial protein expression systems to suit your research needs.

(*) Protein Expression Service: Failure fee may apply, highly dependent on the actual project and target protein.

E.coli

E.coli

Service description

• Codon optimisation and gene synthesis
• Subcloning of gene in T7 promoter-based expression vectors
• Transform construct into 3 different E coli strains
• Screening & expression pilot: cystoplasm or inclusion bodies
• Scale up expression and purification: 1-10 L fermentation
• Affinity purification (Ni2+, GST, etc)
• Refolding (inclusion bodies)
• QC: SDS-PAGE analysis, western blot, purity, concentration
• Project report

Lead time: 7-8 weeks

Yeast

Yeast

Service description

• Codon optimisation and gene synthesis
• Subcloning of gene in yeast expression vector
• Transform construct into E coli strain
• Prepare and linearise plasmid and transform 3-5 yeast strains
• Small scale expression, screening & expression pilot
• Scale up culture and purification
• Affinity purification (Ni2+, GST, etc)
• QC: SDS-PAGE analysis, western blot, purity, concentration
• Project report

Lead time: 9-10 weeks


Remarks:
Pichia pastoris expresses a very low amount of secreted proteins meaning the majority of proteins in the media represent the recombinant protein of interest. This property clearly simplifies the downstream protein purification process.
Pichia pastoris can be grown at high cell density. Thus,this host organism allows for high yield protein production.
Pichia pastoris possesses the complete post-translational machinery characteristic of eukaryotic cells. Thus, this host organism is able to perform phosphorylations and glycosylations (different from those observed in mammalian cells protein expression).

To conclude, Pichia pastoris protein expression is the first choice for the production of secreted proteins.

Insect cells - baculovirus

Insect cells - baculovirus

Service description

• Codon optimisation and gene synthesis
• Subcloning in baculovirus transfer vector
• Recombinant bacmid generation and preparation
• Insect cells transfection and stock virus generation
• Pilot Expression
• Expression and Purification
• Affinity purification (Ni2+, GST, etc)
• QC: SDS-PAGE analysis, western blot, purity, concentration
• Project report

Lead time: 9-10 weeks

Mammalian cells

Mammalian cells

Service description

• Codon optimisation and gene synthesis
• Subcloning of gene in CMV promoter-based expression vectors
• Transform construct into E coli strain
• Prepare plasmid and transfect transient cells strains
• Screening for expression
• Scale up culture cells for transfection
• Affinity purification (Ni2+, GST, etc)
• QC: SDS-PAGE analysis, western blot, purity, concentration
• Project report

Lead time: 7-8 weeks

Proteinexpression

Bitte geben Sie hier ihre zu synthetisierende DNA-Sequenz (5´-3´) ein.

Bitte geben Sie an für welchen Organismus die Sequenz optimiert werden soll. Bitte geben Sie auch an, falls bestimmte Restriktionsschnittstellen vermieden werden sollen.

Bitte geben Sie an, welches Proteinexpressionssystem Sie bevorzugen.

Bitte geben Sie hier z.B. an, welche Reinheitsstufe und welche Menge des Proteins benötigt werden.

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