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Peptidmodifikationen

K-Biotin , Lys-N-epsilon-Biotin & more

Non-standard residues

Aminoisobutyric acid, [aib]
Azido-Lysin, [K-n3] **)
Beta aspartic acid, [beta D]
Beta homo amino acids, [beta homo-aa]
Beta-alanine, [beta A]

Citrullin, [cit]

4-Carboxyglutaminsäure, Gla, (HOOC)2CH-CH2-CH(NH2)-COOH

Cyclohexylalanine, [cha]
D amino-acids, [d-aa]
Dihydroxyphenylalanine, [dopa]
Epsilon lysine, [epsilon K]
Fluoro-, chloro-, bromo phenylalanine, [x-F]
Fluoro-, chloro-, bromo tyrosine, [x-Y]
Gamma glutamic acid [gamma E]

Hydroxyproline, [hyp]
K-Biotin , Lys-N-epsilon-Biotin
Methyl-, acetyl-lysine [meK], [acK]
N-methyl amino-acids, [N-Me-aa]
Norleucine, [nle]
Pencillinamine, [pen]
Phosphorylation [pS], [pT], [pY]



**) Azides of amino acids can be labeled with terminal Alkyne or strained Alkyne (e.g. DBCO)-tagged reporter molecules via a Cu(I)-catalyzed Alkyne-Azide (CUAAC) or Cu(I)-free strain-promoted Alkyne-Azide Click Chemistry (SPAAC) reaction, respectively.

Some examples for possible applications:

Furin substrate: L-Pyr-Arg-Thr-Lys-Arg-AMC (AMC = 7-Amino-4-methylcoumarin).

Heavy isotope (13C, 15N) labeled amino-acids

13C15N-K
13C15N-R
13C15N-A
13C15N-I
13C15N-L
13C15N-F
13C15N-P
13C15N-V


Methyl amino acids
ADMA (Arginine)
doi: 10.2174/157340310791162659
PMCID: PMC2892080
PMID: 21532773
The Role of Asymmetric Dimethylarginine (ADMA) in Endothelial Dysfunction and Cardiovascular Disease
{Lys(Me)}
{Lys(Me2)}
{Lys(Me3)}
N-Methyl amino acid
{N-Me-Ala}
{N-Me-Phe}
{N-Me-Leu}
{N-Me-Ile}
{N-Me-Val}
{N-Me-Met}
{N-Me-Nle}
{N-Me-Nva}
{Sar}
{N-Me-Ser}
{N-Me-Tyr}
{N-Me-Thr}
{N-Me-Asp}
{N-Me-Glu}
N-Me-beta-Ala

N- and C-terminal modifications

Acetyl, formyl, [ac], [for]
Aminooxyacetyl, [aoa]


Azide, [N3]
N-terminal KLH (keyhole limpet hemocyanin) conjugation
Pyroglutamate, [pyr]
S-acetylthioacetate, [sata]
Pyruvat, pyruvic acid [pyruv]. (CH3COCOamide-AA)
Succinyl, [suc]

C-terminal modification
Amide, [NH2]
Alkylester, [COOMe], [COOEt]
Cysteamide, [cya]
Ethylenediamine, [ed]
Hydroxysucinimide ester, [Osu]
C-terminal KLH (keyhole limpet hemocyanin) conjugation
N-methylamide, [NHMe]

IgG antibodies conjugation: IgG-FITC, IgG-HRP, IgG-Biotin

Internal & terminal Spacer
C6 spacer
Chemical name is: 6-aminohexanoic acid NH2-(CH2)5-COOH,
Synonym: AHX.

Chelator
1,4,7,10-Tetraazacyclododecan-1,4,7,10-tetraessigsäure,
Synonym: DOTA

Zugriffe heute: 5 - gesamt: 6120.

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