Aviäre IgY Antikörper (Chicken)
50-100 mg total IgY purified antibodies derived from yolk (eggs). ELISA, guaranteed titer >1:20 000 from purified egg yolk.
|Cat. No. HP1AB05-01 Polyclonal Egg Yolk Antibodies|
|Purification: Total IgY purification|
|Host: 2 Hens|
|Antigen Preparation||Peptide Synthesis (up to 20 aa) & optimized conjugation to a carrier KLH.|
|Chicken Immunisation||4 immunisations, 70 days ; Egg collection **) This procedures can be continued and prolongated on demand (additional fee).|
|Purifications||Yolk isolation and total IgY purification|
|First Deliveries **)||50-100 mg total IgY purified from egg yolk. Pre-immune purified total IgY. 2-3 mg free peptide, purity >90%|
|Report with ELISA data ; Quantitation ; ELISA, guaranteed titer >1:20 000 from purified egg yolk, LC & MS analysis|
Polyclonal chicken antibodies (IgY) for research.
Why Chicken affinity purified IgY?
- Because, higher titres against highly-conserved mammalian gene products!
Easier double immunostaining
Chicken IgYs can be used together with mouse and rabbit antibodies, without the danger of cross-reactivity. Secondary antibodies against chicken IgYs don’t cross-react with mammalian IgGs.
Higher detection signals
Chicken IgYs contain a larger glycosylation index, allowing more labeling with HRP and other antibody tags. This produces higher detection signals.
No "Fc domain" within the stem portion of a chicken IgY
This provides several advantages over rabbit IgGs:
It reduces the likelihood of having false positives in diagnostic applications, since it is the mammalian Fc domain that binds "rheumatoid factors" or other naturally occurring anti-Fc antibodies.
Does not activate mammalian complement systems, allowing the use of chicken IgYs in in vivo applications.
Does not bind to mammalian Fc receptors, avoiding non-specific binding to cells expressing such receptors (e.g., macrophages and dendritic cells).
Zugriffe heute: 1 - gesamt: 1968.