Aviäre IgY Antikörper (Hen)
- Host: Huhn
IgY-Antikörper aus Hühnereiern
| Cat. No. HP1AB05-01 Polyclonal Egg Yolk Antibodies | Inquiry |
| Purification: Total IgY purification | |
| Host: 2 Hens | |
| Antigen Preparation | Peptide Synthesis (up to 20 aa) & optimized conjugation to a carrier KLH. |
| Chicken Immunisation | 4 immunisations, 70 days ; Egg collection **) This procedures can be continued and prolongated on demand (additional fee). |
| Purifications | Yolk isolation and total IgY purification |
| First Deliveries **) | 50-100 mg total IgY purified from egg yolk. Pre-immune purified total IgY. 2-3 mg free peptide, purity >90% |
| Report with ELISA data ; Quantitation ; ELISA, guaranteed titer >1:20 000 from purified egg yolk, LC & MS analysis | |
| Price |
Bestellnummer: HP1AB05-01 Preis: 956 € zzgl. VAT **) Weitere optionale Lieferungen werden separat berechnet. Versandkosten: 55€ |
IgY chicken antibodies
50-100 mg total IgY purified antibodies derived from yolk (eggs). ELISA, guaranteed titer >1:20 000 from purified egg yolk.
Bestellnummer: HP1AB05-01
Chicken IgY Antibodies
In many instances, raising antibodies in chicken has proven essential. Where genetically distant species is required e.g. conserved mammalian proteins, Genosphere Biotechnologies will produce your antibodies in chicken. IgY antibodies may be isolated from circulating blood of chicken or extracted and purified in high yields from egg yolks of immunized laying hens.
Anti-peptide service offer:
• Synthesis, purification & KLH conjugation of your peptide
• Immunization of 2 hens
• Eggs collection, yolk isolation or antiserum collection and IgY purification
√ ELISA titered IgY antibodies fraction
√ Pre-immune purified IgY
√ Full report with ELISA analysis data
• 2 mg of unconjugated peptide (with HPLC & MS data, purity >90%)
Anti-protein service offer (you provide the antigen):
• Immunization of 2 hens
• Eggs collection, yolk isolation or antiserum collection and IgY purification
√ ELISA titered IgY antibodies fraction
√ Pre-immune purified IgY
√ Full report with ELISA analysis data
Pre-immune eggs are collected and control IgYs are purified. Following four immunizations over a 70-days period, eggs or antiserum are collected and we isolate the IgY fraction (purity 90-95%). The final antibodies preparation is then titered by ELISA. In addition, the total IgY fraction may be further purified by affinity chromatography using a peptide- or antigen-column to yield a specific hen antibody. For large scale IgY production, multiple hen immunization, and timely supply over long periods, please contact us with your project details and we will design the most efficient strategy for you.
Inquiries: info@genosphere-biotech.de
Polyclonal chicken antibodies (IgY) for research.
Why avian Chicken affinity purified IgY ?
- Because, higher titres against highly-conserved mammalian gene products !
Easier double immunostaining
Chicken IgYs can be used together with mouse and rabbit antibodies, without the danger of cross-reactivity. Secondary antibodies against chicken IgYs don’t cross-react with mammalian IgGs.
Higher detection signals
Chicken IgYs contain a larger glycosylation index, allowing more labeling with HRP and other antibody tags. This produces higher detection signals.
No "Fc domain" within the stem portion of a chicken IgY
This provides several advantages over rabbit IgGs:
It reduces the likelihood of having false positives in diagnostic applications, since it is the mammalian Fc domain that binds "rheumatoid factors" or other naturally occurring anti-Fc antibodies.
Does not activate mammalian complement systems, allowing the use of chicken IgYs in in vivo applications.
Does not bind to mammalian Fc receptors, avoiding non-specific binding to cells expressing such receptors (e.g., macrophages and dendritic cells).
Antibody glossary
Int Immunopharmacol. 2019 Aug; 73: 293–303.
Published online 2019 May 22. doi: 10.1016/j.intimp.2019.05.015
PMCID: PMC7106195
PMID: 31128529
Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review
E.P.V. Pereira,⁎ M.F. van Tilburg, E.O.P.T. Florean, and M.I.F. Guedes
Pigeon Tektin-5 (TEKT5)
Zugriffe heute: 7 - gesamt: 1443.