Seamless cloning kit - Splicing method

One-Step Seamless Cloning Kit is designed for the simultaneous and directional cloning of PCR fragments into any linearized vector within a single incubation step. This kit is particularly useful for creating custom plasmids with a variety of sequences and sizes.

The kit utilizes a enzymatic mixture to assemble DNA fragments with terminal homology without leaving additional sequences or gaps, ensuring a seamless cloning process. The end homology can be easily integrated using PCR amplification with specific DNA oligos . The success of the cloning is independent of the insert sequence and vector type, allowing for the creation of nearly any desired sequence or sequence combination that can be linearized by restriction digestion or PCR amplification

IgY from Yolk * Genosphere Biotechnologies

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